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Corticosterone enhances CSD susceptibility via glucocorticoid receptor activation in familial hemiplegic migraine 1 Cacna1a knock-in mice
The Journal of Headache and Pain volume 14, Article number: P85 (2013)
FHM1 mutant mice carrying the R192Q gain-of-function mutation in CaV2.1 (P/Q-type) calcium channels display enhanced glutamatergic transmission and increased propensity for cortical spreading depression (CSD;1,2). Corticosteroids released after stress also enhance glutamatergic transmission but the relationship between stress and migraine is not well understood.
We aimed to investigate the acute effects of corticosterone and the role of GR activation on CSD susceptibility in FHM1 R192Q knock-in mice.
Corticosterone (20 mg/kg) or vehicle was injected subcutaneously 4 hours before CSD frequency recordings were carried out in FHM1 R192Q mice. A subgroup of mice was injected with the glucocorticoid receptor antagonist mifepristone 50 minutes before corticosterone/vehicle injection.
Corticosterone injection increased CSD frequency in FHM1 mice compared to vehicle-injected controls but not in wild-types. Pretreatment with mifepristone reduced CSD frequency to the level of vehicle-injected controls. Baseline corticosterone plasma levels were similar in WT and FHM1 mice, while 3 hours after corticosterone administration corticosterone plasma levels were strongly elevated to comparable levels in both WT and FHM1 mice.
These data suggest that combined effects of glucocorticoid receptor activation and the FHM1 R192Q CaV2.1 gain-of-function mutation on excitatory neurotransmission may play a role in proposed effects of stress on migraine attacks.
van den Maagdenberg AMJM, et al.: A Cacna1a knockin migraine mouse model with increased susceptibility to cortical spreading depression. Neuron 2004, 41: 701–710. 10.1016/S0896-6273(04)00085-6
Tottene A, et al.: Enhanced excitatory transmission at cortical synapses as the basis for facilitated spreading depression in Ca(v)2.1 knockin migraine mice. Neuron 2009, 61: 762–773. 10.1016/j.neuron.2009.01.027
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Shyti, R., Eikermann-Haerter, K., Meijer, O. et al. Corticosterone enhances CSD susceptibility via glucocorticoid receptor activation in familial hemiplegic migraine 1 Cacna1a knock-in mice. J Headache Pain 14, P85 (2013). https://doi.org/10.1186/1129-2377-14-S1-P85
- Migraine Attack
- Cortical Spreading Depression