Fig. 4
Effect of NBP on CGRP and c-Fos expression in SP5C. A, B CGRP and c-fos immunofluorescence staining. CGRP immunofluorescence intensity and the number of c-fos-positive cells were significantly increased in the NTG + Oil group compared with the Control + Oil group, and CGRP immunofluorescence intensity and the number of c-fos-positive cells were significantly decreased in the NTG + NBP group compared with the NTG + Oil group. C, D Protein expression of CGRP and c-Fos. CGRP and c-Fos protein levels were significantly higher in the NTG + Oil group compared to the Control + Oil group. CGRP and c-Fos protein levels were significantly lower in the NTG + NBP group compared to the NTG + Oil group. E Expression of CGRP in plasma. Plasma CGRP expression was significantly higher in the NTG + Oil group compared to the Control + Oil group and significantly lower in the NTG + NBP group compared to the NTG + Oil group. (n = 6–8 in each group; scale bar = 50/100 μm; **p < 0.01 and ****p < 0.0001 compared with the Control + Oil group; #p < 0.05 and ##p < 0.01 compared with the NTG + Oil group)