Fig. 3

Effects of NBP on mechanical thresholds, photophobia, and anxiety. A, B Compared with the NTG + Oil group, NBP at 30 mg/kg, 60 mg/kg, and 120 mg/kg significantly protected the hindpaw and periorbital mechanical thresholds in mice. C Compared with the NTG + Oil group, 30 mg/kg, 60 mg/kg, and 120 mg/kg NBP significantly increased the time spent in the light box and the number of times the mice entered the lightbox. D Compared with the NTG + Oil group, 30 mg/kg, 60 mg/kg and 120 mg/kg of NBP significantly increased the time spent in the open arm and the number of entries into the open arm. (n = 8–10 in each group; p < 0.01,p < 0.0001 the NTG + NBP (30mg/kg) group compared with the NTG + Oil group; *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 compared with the Control + Oil group; #p < 0.05, ##p < 0.01, ###p < 0.001 compared with the NTG + Oil group)