Fig. 1

Timing of drug administration, behavioral testing and sampling, and behavioral paradigms. A The Control and NTG groups. B The Control + Oil, NTG + Oil and NTG + NBP groups. C The Control + Oil + ML385, NTG + Oil + ML385, NTG + NBP + ML385 and NTG + NBP + Vehicle groups. Tissue collection was performed within 24 h after the last NTG injection, photophobia, and elevated-plus maze experiments were performed within 24 h after the last NTG injection, and mechanical thresholds were performed 30 min before each NTG injection. D Schematic representation of mechanical threshold, photophobia, and anxiety behaviour tests