Fig. 9
Ca2+ response and CGRP upregulation evoked by the activation of TRPA1 and TRPV1 are restrained by 2R, 6R-HNK. a Statistical donut charts indicated the effect of 2R, 6R-HNK pretreatment on the ratio of responding neurons to sequential stimuli of AITC-and-capsaicin with 80-s interval. b Statistics graphs of Ca2+ responses in small, medium and large DRG neurons with 2R, 6R-HNK preincubation or not, ****p < 0.0001 compared with HHBS group. c-d After calcium imaging, representative images of immunofluorescence (c) and cytometry (d) showed the changes of excitability and CGRP expression in DRG neurons after concomitant administration of AITC-and-capsaicin with or without 2R, 6R-HNK pretreatment (n = 5 samples/group), *p < 0.05, **p < 0.01 compared with each groups