Fig. 7

The increases in TRPA1/TRPV1 expression and their co-expression with CGRP are reduced by 2R, 6R-HNK. a RT-qPCR results showing the mRMA expressions mRNA in bilateral DRGs or SDH at 3 h/1 d after LF-PENS from different groups (n = 5 ~ 7 mice/group). b-g Representative photomicrographs of immunofluorescent (b, e), statistical analysis (c, f) and double-labeling staining (d, g, magnified from the white dotted boxed in the monochromatic micrographs b, e) and illustrated the changes of TRPA1, TRPV1 and the co-expression with CGRP in bilateral L4 DRGs at 3 h or 30 d after LF-PENS (n = 5 mice/group, 4 sections/mouse). h, i Western blot and statistical analysis showed the protein expressions in DRG and SDHs in each group (n = 3 mice/group). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001 between groups