Fig. 6

LF-PENS-induced CGRP overexpression rather than microglial activation is suppressed by intrathecal 2R,6R-HNK. a PCR cycle quantification (Cq) value of CGRPα mRNA (Calcα) was lower than β (Calcβ) in Saline 1 d L4 DRGs (n = 6 or 8 mice/group). b RT-qPCR analysis of Calcα, β, and Bdnf mRNA in bilateral DRGs at 3 h from different groups (n = 5 ~ 7 mice/group). c-i Representative immunofluorescent images and statistical analysis demonstrated that CGRP upregulation but not microglial activation in bilateral L4 DRGs and L4 ~ 5 SDH at 3 h (c, g) or 30 d (e, h) after LF-PENS was inhibited by 2R, 6R-HNK (n = 5 mice/group, 4 sections/mouse). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001 between groups