Fig. 8
UA ameliorated allodynia in mice by reducing mitochondrial impairment and improving mitochondrial function. A Flow chart of the experiment. B-D The basal mechanical pain thresholds of the hind paw (B), periorbital area (C) and the thermal pain threshold of the hind paw (D) during the injection of UA and NTG. Two-way ANOVA with the Bonferroni post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared with the SHAM + VEH group; #p < 0.05, ##p < 0.01 compared with the NTG + VEH group. E Comparison of mitochondrial morphology in VPM of the thalamus between the NTG + VEH and NTG + UA groups. a and b Representative micrographs of mitochondria in VPM of the NTG + VEH group, Scale bar, 5 μm (a) and 2 μm (b). c and d Representative micrographs of mitochondria in VPM of the NTG + UA group, Scale bar, 5 μm (c) and 2 μm (d). Abnormal mitochondria were indicated by red arrows. F The number of abnormal mitochondria per 100 µm2 is calculated in two groups. Independent-sample t test. **p < 0.01. G After UA treatment, concentration of MDA induced by NTG administration decreased. One-way ANOVA and Tukey’s multiple comparison test. ****p < 0.0001: the NTG + VEH group vs. the SHAM + VEH group, **p < 0.01: the NTG + VEH group vs. the NTG + UA group. n = 6 per group. All data are presented as the mean ± SEM