Fig. 2

Acute LVC induced fast and brief periorbital and hind paw mechanical hyperalgesia. Both LVC and NTG injection significantly reduced mechanical thresholds in both periorbital (A) and hind paw plantar areas (B) [F(2,17) = 5,464; p < 0,05;]. LVC administration yielded a rapid reduction at 30 min and this hypersensitivity peaked at 60 min. LVC-induced hypersensitivity ended at 120 min. In comparison, NTG decreased thresholds in 120 min and resulted in longer term hypersensitivity that did not recover until 240 min. Data were shown as mean ± SEM. Statistical analysis was performed with two-way ANOVA and posthoc Tukey’s test (*p < 0,05 LVC vs CTRL; ** p < 0,01 LVC vs. CTRL; † p < 0,05 NTG vs CTRL; # p < 0,05 LVC vs NTG) (n = 6–8/group)