Fig. 5
Interventions on TNC-VN circuit in chronic-NTG treated rat model. A Schematic showing the site of AAV-EF1α-DIO-hM4D-mCherry injection into the unilateral TNC, and the site of AAVretro-Syn-Cre injection into the VN at the same site of chronic-NTG treated rat. B Expression of AAV-EF1α-DIO-hM4D-mCherry (red) in trigeminovestibular neurons. White dashed line circle showing the TNC region. Scale bar, 1000 μm. C Schematic illustration of intervention protocol. Basal responses of periorbital (D) and hind paw (E) mechanical, and thermal (F) thresholds among three groups. Post-treatment responses of periorbital (G) and hind paw (H) mechanical, and thermal (I) thresholds among three groups. Balance beam walk (J) and negative geotaxis test (K) among three groups. N = 6 rats/group. Two-way ANOVA with the Bonferroni post hoc test, *p < 0.05 compared with saline, **p < 0.005 compared with saline, ***p < 0.001 compared with saline