Fig. 1
Dissection of GlutamateTNC-CGRPVN circuit. A (Left) Schematic showing the site of FG injection into the unilateral VN of WT rat. (Right) Representative image of FG injection site in VN at transverse plane. White dashed line circle showing VN region. Scale bar, 500 μm. B Representative images showing the co-localization of Glutamate (upper row, green) or GABA (lower row, red) and FG (gold) in TNC. Arrowhead indicates co-localized neurons. Scale bar, 50 μm. C Percentage of glutamate + /FG + neurons among glutamate + neurons (present as green dot), and GABA + /FG + neurons among GABA + neurons (present as red triangle) in TNC. N = 5 rats/group, each dot represents the average data of one rat. Mann–Whitney U test, ***p < 0.0001. D (Left upper row) Schematic showing the site of H129-G4 injection into the unilateral TNC of WT rat. (Left lower row) Representative image of H129-G4 injection site in TNC at sagittal plane. White dashed box showing the injection site. Scale bar, 2000 μm. (Right) Representative image of H129-G4 injection site in TNC at transverse plane. White dashed line circle showing TNC region. White box showing the injection site. Scale bar, 500 μm. E Representative images showing the co-localization of H129-G4 (green) and CGRP (red) in VN. White dashed line circle showing VN region. Scale bar, 500 μm. F Magnified view of E image. Scale bar, 50 μm