Table 1 Antibodies used in western blot and immunofluorescence staining assays
Antibody | Manufacturer | Number | Host | Dilution |
|---|---|---|---|---|
For western blot analysis | ||||
 PACAP | Santa Cruz | sc-166180 | Mouse | 1:500 |
 PAC1 | Abclonal | A3120 | Rabbit | 1:1000 |
 c-Fos | Proteintech | 66,590–1-Ig | Mouse | 1:2000 |
 PSD95 | Affinity Biosciences | AF7839 | Rabbit | 1:1000 |
 SYP | Wanleibio | WL03058 | Rabbit | 1:1000 |
 SYT1 | Abclonal | A0992 | Rabbit | 1:1000 |
 Phospho-ERK1/2 (Thr202/Tyr204) | Affinity Biosciences | AF1015 | Rabbit | 1:1000 |
 ERK1/2 | Affinity Biosciences | AF0155 | Rabbit | 1:1000 |
 Phospho-CREB (Ser133) | Affinity Biosciences | AF3189 | Rabbit | 1:1000 |
 CREB | Affinity Biosciences | AF6188 | Rabbit | 1:1000 |
 BDNF | Wanleibio | WL0168 | Rabbit | 1:500 |
 GAPDH | Proteintech | 10,494–1-AP | Rabbit | 1:3000 |
 HRP conjugated anti-mouse | Servicebio | GB23301 | Goat | 1:3000 |
 HRP conjugated anti-rabbit | Servicebio | GB23303 | Goat | 1:3000 |
For immunofluorescence staining assays | ||||
 PACAP | Santa Cruz | sc-166180 | Mouse | 1:50 |
 PAC1 | Abcam | ab28670 | Rabbit | 1:500 |
 c-Fos | Servicebio | GB12069 | Mouse | 1:500 |
 GFAP | Wanleibio | WL0836 | Rabbit | 1:100 |
 NeuN | Proteintech | 26,975–1-AP | Rabbit | 1:300 |
 Iba1 | Wako | 019–19,741 | Rabbit | 1:500 |
 PSD95 | Affinity Biosciences | AF7839 | Rabbit | 1:200 |
 Dylight 594, Goat Anti-Rabbit IgG | Abbkine | A23420 | Goat | 1:200 |
 Dylight 594, Goat Anti-Mouse IgG | Abbkine | A23410 | Goat | 1:200 |
 DyLight 488, Goat Anti-Mouse IgG | Abbkine | A23210 | Goat | 1:200 |
 Dylight 488, Goat Anti-Rabbit IgG | Abbkine | A23220 | Goat | 1:200 |