Fig. 2

The protein expression and fluorescence localization of PACAP and PAC1R in different groups. A-D. Representative immunoblots and densitometric quantification of PACAP (A) and PAC1R (C) in the trigeminal nucleus caudalis (TNC). B. Representative microphotographs of co-immunofluorescence staining of PACAP (red) with neurons (NeuN, green), astrocytes (GFAP, green), and microglia (Iba1, green) in TNC after CM. The nuclei were stained with DAPI (blue). D. Representative microphotographs of co-immunofluorescence staining of PAC1R (red) with neurons (NeuN, green) and PSD95 (green) in TNC after CM. The nuclei were stained with DAPI (blue). Scale bar: 50 μm. Mean ± SEM, n = 3/group, two-tailed Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001 compared with saline group. Abbreviations: NTG, nitroglycerin