Fig. 7
Overexpression of Sirt3/Pgc-1α enhanced expression of each other and improved mitochondrial function in PC12 cells. PC12 cells infected by lentivirus (LV) were treated with/without H2O2 (300 nM) for 12 h. A Western blot analysis of Sirt3 (**p(LV-Sirt3) = 0.0066, *p(LV-Pgc1α) = 0.0311) and Pgc-1α (+p = 0.05; *p(LV-Sirt3) = 0.0242, **p(LV-Pgc1α) = 0.0096). n = 3 per group; Student’s t-test. B-C Flow cytometric analysis was used to detected the ROS levels in different groups. n = 3 per group; Student’s t-test. +p(LV-NC) = 0.0371, +p(LV-Pgc1α) = 0.0158; **p(LV-Sirt3) = 0.0019, ***p(LV-Pgc1α) = 0.0001. D The ATP levels were examined and normalized by total protein concentrations in different groups. n = 3 per group; Student’s t-test. +++p(LV-NC) = 0.0005; *p(LV-Pgc1α) = 0.0295. Data are represented as Mean ± SD; *p < 0.05, **p < 0.01 and ***p < 0.001 as compared to LV-NC group. +p < 0.05 and +++p < 0.001 as compared to LV- group. NC, negative control. NS, no significance