Fig. 4
SS-31 restored mitochondrial function and mitochondrial homeostasis in H2O2-induced PC12 cells. A PC12 cells were treated with SS-31 (100 nM) for 2 h, followed by additional H2O2 (300 nM) for 12 h. B Western blot analysis of Sirt3 and Pgc-1α (F(2, 6) = 7.438, *p = 0.0237; *p = 0.0452, +p = 0.0292). Protein levels of Sirt3 and Pgc-1α were quantified normalized to β-actin. n = 3 per group; One-way ANOVA. (C) Western blot analysis of Tfam (F(2, 6) = 6.981, *p = 0.0272; +p = 0.0254), Drp1, Fis1 (F(2, 6) = 8.292, *p = 0.0188; *p(H2O2) = 0.0200, *p(SS-31+H2O2) = 0.0485) and Pink1 (F(2, 6) = 20.93, **p = 0.0020; **p(H2O2) = 0.0037, **p(SS-31+H2O2) = 0.0030). Protein levels were quantified normalized to β-actin. n = 3 per group; One-way ANOVA. D and E Flow cytometric analysis was used to examine MMP (F(2, 6) = 16.90, **p = 0.0034; ++p = 0.0045) and ROS (F(2, 6) = 7.564, *p = 0.0229; *p(H2O2) = 0.0314, *p(SS-31+H2O2) = 0.0388) levels in different groups. n = 3 per group; One-way ANOVA. Data are represented as Mean ± SD; *p < 0.05 and **p < 0.01 as compared to con group. +p < 0.05 and ++p < 0.01 as compared to H2O2 group