Fig. 4

GTN alters NPY expression in several brain areas, including the medial habenula (MHb). A The representative reconstructed NPY (green) images from a VEH or GTN mouse brain in diverse brain areas. Each group's left panel is the overview (scale bar, 1000 μm), and higher magnification close-ups are shown in the right panel (scale bar, 100 μm). The white contour delineates the borders of the respective brain areas. B Summary fold changes in the number of NPY neurons of the GTN group over the VEH group in multiple brain areas. n = 6 mice per group. Significance was assessed using a two-tailed unpaired Student’s t-test. Data are presented as the mean ± S.E.M. *P < 0.05, **P < 0.01. MHb, medial habenula; SPVO, spinal nucleus of the trigeminal oral part; SPVC, spinal nucleus of the trigeminal caudal part; GU5, gustatory areas layer 5; SSp-n5, primary somatosensory area nose layer 5; SSp, primary somatosensory area; SSs, supplemental somatosensory area; VPM, ventral posteromedial nucleus of the thalamus; LP, lateral posterior nucleus of the thalamus; NPC, nucleus of the posterior commissure; PGRN, paragigantocellular reticular nucleus; NTS, nucleus of the solitary tract