Fig. 2
TAAR1 mediates the tyramine-induced reduction in IA. a Western blot analysis of TAAR1 and TAAR4 protein expression in mouse TGs. β-actin was used as an internal loading control. The immunoblots are representative of the results of at least three independent experiments. b Colocalization of TAAR1 (red) with NeuN, GS, IB4, CGRP or NF200 (green) in the TG (arrows). Scale bar = 50 μm. c and d The time course curve (c) and the bar chart (d) showing that pretreatment of TG neurons with EPPTB (3 µM) prevented the 0.1 µM tyramine-induced IA decrease (n = 10 cells). EPPTB at 3 µM alone did not affect IA (n = 7 cells). Insets in panel c indicate the representative traces. The letters indicate the points used for sample traces. **p < 0.01 vs. tyramine, unpaired t test. e Summary of results indicating quantified IA current density under control conditions, during exposure to RO5263397, and during washout (n = 9 cells). *p < 0.05 vs. control, paired t test. f Western blot analysis of TAAR1 protein expression in the NC-siRNA or TAAR1 siRNA-treated (TAAR1-siRNA) groups. The immunoblots are representative of the results of at least three independent experiments. **p < 0.01 vs. NC-siRNA, unpaired t test. g Representative traces (left panel) and bar chart (right panel) showing that treatment with TAAR1-siRNA abrogated the 0.1 µM tyramine-induced IA response (n = 12 cells). *p < 0.05 vs. control + NC-siRNA, unpaired t test