Fig. 2

Systemic U-69,593 increased water consumption and urination in female and male mice. Mice were individually placed in metabolic chambers and received a single subcutaneous administration of U-69,593 at 30 mg/kg or vehicle-control. A Water consumption and B volume of urine, as outcome measurements of thirst and polyuria, respectively, were evaluated for 120 min after treatment. C Linear regression was performed to evaluate the correlation between water consumption and urination in U-69,593-treated mice. Female and male data were combined. Data are presented as mean ± SEM and analyzed using one-way ANOVA followed by Sidak’s multiple comparison test with * representing p < 0.05 compared to the control group (n = 12; 6 females/group and 6 males/group)