Fig. 2

c-Fos activation map of cortical structures in the NTG-induced chronic migraine model. A. Representative pictures of c-Fos (red) and NeuN (green) immunofluorescence labeling in the mPFC. Scale bars =100 μm. B-C. mPFC showed a significant increase of c-Fos⁺ cells density (B) and the percentage of c-Fos + cells in NeuN ⁺ cells (C), alleviated by OLC, n = 5/group. D. Representative pictures of c-Fos (red) and NeuN (green) immunofluorescence labeling in the ACC. Scale bars =100 μm. E–F. ACC showed a significant increase of c-Fos⁺ cells density (E) and the percentage of c-Fos⁺ cells in NeuN cells (F), alleviated by OLC, n = 5/group. One-way ANOVA with the Tukey post hoc tests, * P < 0.05, **P < 0.01. Abbreviations: mPFC, medial prefrontal cortex; ACC, anterior cingulate cortex; OLC, olcegepant; NTG, nitroglycerin