Table 1 Primary and secondary antibodies used
Product | Code | Source | Dilution | Immunogen | Company |
CB1 | ACR-001 | Rabbit | 1:100 | N-terminal of rat CB1 receptor | Alomone Labs, Jerusalem, Israel |
CB2 | ACR-002 | Rabbit | 1:100 | 3rd intracellular loop of rat CB2 receptor | Alomone Labs, Jerusalem, Israel |
CGRP | Ab81887 | Mouse | 1:100 | Rat α-CGRP | Abcam, Cambridge, UK |
RAMP1 | 844 | Goat | 1:100 | C-terminal of human RAMP1 | Merck & Co., Inc., West Point, PA, USA |
Product | Source | Dilution | Against | Company | |
FITC | Goat | 1:100 | Anti-rabbit | Cayman Chemical, Ann Arbor, MI, USA | |
Alexa 594 | Goat | 1:100 | Anti-mouse | Jackson Immunoresearch Laboratories, Inc., West Grove, PA, USA | |
Cy3 | Donkey | 1:200 | Anti-goat | Jackson Immunoresearch Laboratories, Inc., West Grove, PA, USA | |