Fig. 3

The mRNA levels of GFAP and Iba1 in the TNC. A Representative images of RNAscope ISH results for GFAP and Iba1 in the TNC of different groups. Blue dots: GFAP. Red dots: Iba1. Purple: cell nucleus. Scale bar: 20 μm. B, C The histograms present the quantitative analysis of the mRNA levels of GFAP and Iba1 in the TNC. One-way ANOVA and Dunnett’s t test post hoc analysis. n = 4/group, *p < 0.05, **p < 0.01, ***p < 0.001 versus CON