Fig. 5

Pump current recordings of Na⁺/K⁺-ATPase transfected in HEK293T cells. a and b Continuous recordings with wild-type pumps in HEK293T cells. 1 µM ouabain was added to block endogenous pumps. The pump current was activated by superfusion of 20 mM K⁺ and inhibited by 10 µM ouabain. c Representative raw currents of WT (blue), G762S (yellow), R938P (green) and G615R (red) in response to 200 ms steps with + 20 mV increments from − 100 to + 100 mV. d Coexpression of ATP1A2 (green) and ATP1B1 (red) in transfected HEK293T cells. e Ouabain-sensitive pump currents measured in the last 50 ms of test pulses (two-way ANOVA: P = 0.040) (amplitude at + 40 mV, one-way ANOVA compared with WT, n = 9; G762S, n = 10, P = 0.018; R938P, n = 8, P = 0.142; G615R, n = 10, P = 0.008)