Fig. 3

Na⁺/K⁺-ATPase activity test. a-c Na⁺/K⁺-ATPase activity of each mutant in the absence and presence of 10 µM or 100 µM ouabain. The activity of the Na⁺/K⁺-ATPase was normalized to that of the WT Na⁺/K⁺-ATPase without ouabain. d Na⁺/K⁺-ATPase activity with and without ouabain. Mutations were divided into three groups according to their FHM phenotypes. e The ouabain-sensitive ATPase activity of the WT pump was higher than that of mutant pumps in the presence of 10 µM and 100 µM ouabain. P value compared with the WT: ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, Kruskal-Wallis test. P value between groups: *P < 0.05, **P < 0.01, ***P < 0.001, Kruskal-Wallis test