Table 1 Schematic representation of experimental groups, sample size (number, n) and the sampling time (hour, h) of mice per group for different experimental analysis

Sham

9

-

6

-

3

-

24h after the first, the second, the third and the last NTG i.p. injections

NTG (1d)

6

-

6

-

-

-

NTG (3d)

6

-

6

-

-

-

NTG (5d)

6

-

6

-

-

-

NTG (9d)

9

-

6

-

3

-

Sham

20

8

6

6

4

4

For c-fos: 2h after the last NTG/saline i.p. injection;

For other targets: 24h after the last NTG/saline i.p. injection

Exe(9-39)

12

8

6

6

-

-

Lira

12

8

6

6

-

-

CM

20

8

6

6

4

4

CM+Exe(9-39)

20

8

6

6

4

4

CM+Lira

20

8

6

6

4

4

CM+LY294002

8

8

-

-

-

-