Fig. 1

Recurrent NTG injection increased GLP-1R expression in the TNC. a The white dotted line frame exhibits the TNC regions. b-d Representative western blot showing changes in GLP-1R and GLP-1 protein expression in the TNC on different days after NTG injection (b). The band intensities are presented as the percent (%) change relative to β-actin (c-d). Data are presented as mean ± SEM; n = 6/group; *p < 0.05, **p < 0.01 vs. sham group. e Representative immunofluorescence images of GLP-1R in the TNC in the sham and NTG (9d) groups. Arrowheads indicate GLP-1R. n = 3/group, scale bar: 20 μm