Fig. 8

Representative images and quantitative analysis of the dendritic spines in the TNC. A and a show the sham group, B and b show the NTG + vehicle group, C and c show the NTG + OT group, D and d show the NTG + OT + L368,899 group. a-d Magnification of the rectangle frame from each group was used to quantify the spine density in the four groups. E Golgi-Cox staining showed that the number of dendritic spines per 20 μm was significantly higher in the NTG + vehicle group than in the sham group. The number of dendritic spines after OT treatment was reduced, while the effect of OT was inhibited by L368,899. One-way ANOVA with Dunnett’s post hoc test, n = 4/group; ***p < 0.001 vs. the sham group; ###p < 0.001 vs. the NTG + vehicle group; &&p < 0.01 vs. the NTG + OT group; scale bars: 20 μm