Fig. 5

Gastrocnemius injection of IL-6 produces no significant facial or hindpaw responses in male and female rats. Rats had facial and hindpaw withdrawal thresholds assessed prior to and following administration of 0.1 ng IL-6 (6 females, 6 males) or vehicle (6 females, 5 males) into the gastrocnemius muscle. Two-way ANOVA followed by Bonferroni posthoc analysis revealed no significant effect of treatment on facial or hindpaw responses in female (A, B) (F (1, 40) = 1.844, p = 0.1821) (F (3, 36) = 0.2904, p = 0.8320) or male (C, D) (no variation among groups) (F (1, 36) = 2.289, p = 0.1390) rats