Fig. 2

Peripheral CGRP administration depresses wheel activity during both the inactive and active phases. In all panels, grey areas represent the active phase of the animals when lights of the facility were turned off (night). Empty symbols represent females, and full symbols represent males. There were no statistical differences between males and females. Number of wheel revolutions by CD1 mice was measured for 2 h starting immediately after treatment administration. A The first cohort of mice was tested at 10 AM first, then at 8 PM a week later. Animals were administered with vehicle (PBS, i.p., n = 7) or CGRP (0.1 mg/kg i.p., n = 8) before being placed in the wheels. B The second cohort of mice was tested at 8 PM first, then at 10 AM a week later. Animals were administered with vehicle (PBS, i.p., n = 7) or CGRP (0.1 mg/kg i.p., n = 8) before being placed in the wheels. C Data accumulated at 10 AM from the two cohorts in A and B was pooled together and analyzed per hour. D Data accumulated at 8 PM from the two cohorts in A and B was pooled together and analyzed per hour. Two-way (factors: time x treatment) ANOVA (interaction and treatment factors ns) for panels A and B (underpowered). Unpaired t-test (*p < 0.05) for panels C and D