| |
T0
|
T1
|
Statistical analysis
|
|---|
|
All patients
|
Responders
|
Non-responders
|
All patients
|
Responders
|
Non-responders
|
TIME
|
GROUP
|
TIME
x
GROUP
|
|---|
|
n
|
28
|
23
|
5
|
28
|
23
|
5
|
–
|
|
CGRP (pg/ml)
|
393.3 ± 242.9
|
322.9 ± 164.1
|
717.1 ± 301.1
|
275.2 ± 251.8
|
251.8 ± 383.2
|
383.2 ± 208.7
|
0.001
|
0.033
|
0.542
|
|
miR-382-5p (RQ)
|
14.6 ± 3.2
|
10.4 ± 1.1
|
33.9 ± 15.6
|
2.2 ± 0.4
|
1.7 ± 0.3
|
4.4 ± 1.1
|
0.001
|
0.009
|
0.344
|
|
miR-34a-5p (RQ)
|
69.5 ± 8.5
|
66.7 ± 8.8
|
81.8 ± 27.2
|
21.6 ± 4.0
|
20.7 ± 4.4
|
25.9 ± 10.4
|
0.001
|
0.775
|
0.804
|
- Legend: CM-MO chronic migraine with medication overuse. Responders: patients with a reduction of at least 30% of monthly migraine days after detoxification. T0: baseline evaluation; T1: 2 months after discharge from in-hospital detoxification. CGRP values are presented as mean ± standard deviation. microRNAs values are presented as “mean ± standard error of the mean”. RQ Relative quantification: 2−∆∆Ct = 2 − (∆Ct gene − ∆Ct housekeeping gene), Ct cycle threshold as defined in methods section. Statistical analysis: non-parametric tests for repeated measures with two factors: factor TIME (T0 vs. T1), and factor GROUP (responders vs. non-responders)
