Fig. 5
Knockdown of calcitonin gene-related peptide (CGRP) in the trigeminal ganglion reduced c-fos and CGRP levels in the trigeminal nucleus caudalis (TNC) and vestibular nuclei (VN) after CM. a Representative images of immunofluorescence staining showing GFP (green) labeled lentivirus was injected into the trigeminal ganglion and dispersed in V1 and V2 branches. Representative images of immunofluorescence staining showing the immunoreactivity of CGRP in the TNC (b) and VN (c). Quantitative assessments showed that pretreatment of lentiviral vectors comprising CGRP short hairpin RNA (LV-CGRP) significantly reduced CGRP immunoreactivity in the TNC (d) and mRNA levels of CGRP in the VN (e). f Representative images showing the c-fos + neurons in the TNC and VN. (g) In the LV-CGRP treated group, the number of c-fos positively stained neurons in the TNC and VN significantly reduced compared with blank lentiviral vectors (LV-NC). FOV = 1.02 × 106 μm3. b, f Scale bar = 50 μm; *P < 0.05 compared with vehicle, n = 8/group. Data are mean ± SEM. Student’s t-test