Fig. 7

Effect of NTG on p38, p-p38MAPK and BDNF levels in the TNC. a Representative images of Western blot results showing the p38, p-p38MAPK and BDNF protein levels from 3 d to 11 d during the NTG injection. b-d The histogram presents the quantitative analysis of p-p38 (b), p38MAPK (c) and BDNF (d) in the TNC. Data are presented as the mean ± SD. One-way ANOVA and Tukey’s multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001 versus VEH. e Representative photographs of immunofluorescence staining for BDNF (green) and microglia (Iba-1, red) in the TNC in the VEH and NTG groups on day 11. Scale bar = 50 μm. f The white box was amplified. Scale bar = 20 μm. The white arrow indicates some BDNF proteins expressed in neurons