Fig. 7

Inhibition of CGRP receptor internalization by monoclonal antagonist antibodies. Time course of triple positive (yellow) CGRP receptor (AA58 = green, RAMP1 = red, CLR-FAP = magenta) trafficking over 24 h (a-c): Treatment with 479 nM AA58 alone (a), in the presence of sustained exposure to 100 nM CGRP (b) and after 5 min exposure to CGRP followed by wash-out of both CGRP and unbound AA58 (c). Time course of CGRP receptor trafficking over 24 h visualized by RAMP1 (red, d, e): Treatment with 479 nM 8E11 alone (d) and with 8E11 in the presence of sustained 100 nM CGRP exposure (e). 8E11 (green) could not be visualized by secondary detection indicating that it had bound to CGRP and was removed during the fixation process (d, e). Scale bars = 12 μm