Fig. 4

CGRP-induced receptor internalization. 24 h time course of CGRP (100 nM) induced internalization of CGRP receptors triple labeled by AA58, RAMP1 and CLR-FAP: Merged images show co-localization in yellow, nuclei are blue. Arrow heads point to endosomal-like vesicles that appear after 10 min of CGRP stimulation and continue to increase over the 24 h time points (a). Individual channels (AA58 = green, RAMP1 = red, CLR-FAP = magenta) are shown in a 0.15 μm optical slice at 5 min (b) and 60 min (c) after CGRP stimulation (CLR-FAP fluorescence is not visible at 0 min). Arrow heads point to endosomal-like vesicles. Scale bars = 12 μm