Fig. 6
Expression and immunofluorescence staining of PSD95, Syp, and Syt-1 in the TNC in each of the five groups. a The expression levels of PSD95 (b), Syp (c), and Syt-1 (d) were significantly increased in the CM group compared with those in the sham group, and no significant difference in the expression of PSD95 was found between the CM and CM + DMSO groups. The expression of PSD95 in CM rats significantly decreased following the administration of PP2 and genistein (n = 6 in each group, five images per animal, *P < 0.05 compared with the sham group, #P < 0.05 compared with the CM + DMSO group). e Immunofluorescence staining for PSD95, Syp, and Syt-1 in the TNC. Semiquantitative analyses showed that the numbers of PSD95 (f) and Syt-1 (h)-positive cells were significantly increased in the CM group compared with those in the sham group; moreover, PP2 and genistein significantly reduced the numbers of PSD95- and Syt-1-positive cells. Semiquantitative analyses showed that the fluorescence intensity of Syp (g) in the TNC was higher in the rats belonging to the CM group than in those of the sham group, and there was no significant difference in the expression of Syp between the CM and CM + DMSO groups. PP2 and genistein treatment decreased the Syp fluorescence intensity (n = 6 in each group, *P < 0.05 compared with the sham group, #P < 0.05 compared with the CM + DMSO group, scale bar = 50 μm)