Fig. 5
Expression levels of CGRP in the TNC and immunofluorescence staining for CGRP and SP in the TNC. a The expression of CGRP was significantly increased in the CM group compared with that in the sham group, and no significant difference in the expression of CGRP was found between the CM and CM + DMSO groups. The expression of CGRP in CM rats was significantly decreased after the administration of PP2 and genistein (n = 6 in each group, five images per animal, *P < 0.05 compared with the sham group, #P < 0.05 compared with the CM + DMSO group). b The bilateral immunoreactivity of CGRP and SP in the TNC was investigated by immunofluorescence staining (the image shows only one side because no difference was found between the two sides). c The fluorescence intensities of CGRP in the TNC were elevated in the rats belonging to the CM groups compared with those in the sham group, and no significant difference in the expression of CGRP was found between the CM and CM + DMSO groups. PP2 and genistein treatment decreased the CGRP fluorescence intensities. d The fluorescence intensities of SP in the TNC were elevated in the rats of the CM groups with those in the sham group, and there was no significant difference in the expression of SP between the CM and CM + DMSO groups. PP2 and genistein treatment decreased the SP fluorescence intensities (n = 6 in each group, *P < 0.05 compared with the sham group, #P < 0.05 compared with the CM + DMSO group, scale bar = 200 μm)