Fig. 1

Glutamate immunoreactivity in the TNC. In case of fresh, intact animals a few homogenously stained neurons were detected in the TNC. Following CFA application on the dura a clear increase in staining intensity and amount of glutamate positive cells can be seen. After saline application on the dura a moderate increase was seen compared to fresh, intact animals. I.p. treatment with KYNA derivate abolished the amount of glutamate positive cells following CFA induced activation, whereas i.p saline treatment had no effect on glutamate reactivity in the TNC. “+” represent a light, “++” moderate, “+++” strong increase in immunoreactivity