Fig. 5
Human SPG immunohistochemistry. a PACAP-38 immunoreactivity was found in some neurons (thin arrows), in fibers (thick arrow) and vessel walls (thin arrow head). Yellow represent autofluorescent lipofuscin in the neurons (thick arrow head). b nNOS immunoreactivity was found in the neurons (thin arrows) and vessel walls (thin arrow head). Thick arrow points at a negative cell. Yellow; autofluorescent lipofuscin (thick arrow head). All images are in the same magnification. c and d 5-HT1B immunoreactivity was found in most neurons (thin arrows), and in some fibers (thin arrow heads) and vessel walls (thick arrow). Asterisk indicates a negative cell. Yellow; autofluorescent lipofuscin (thick arrow heads). e and f 5-HT1D immunoreactivity was seen in neurons (thin arrows) and fibers (thin arrow heads). Yellow; autofluorescent lipofuscin (thick arrow head) g and h SNAP25 immunoreactivity was exclusively found in SGCs (thin arrow heads). Yellow; autofluorescent lipofuscin (thick arrow head). i and j SV2-A immunoreactivity was confined to neurons (thin arrows). Asterisk indicates a negative cell. Yellow; autofluorescent lipofuscin (thick arrow head). The same magnifications are used throughout the panel (c-j)