Fig. 3
Rat SPG immunohistochemistry – double staining. a nNOS and 5-HT1B double staining revealed neurons that expressed either nNOS (thin arrows) or 5-HT1B (asterisk), but also both (thick arrows). Fibers were mostly nNOS positive (thin arrow head). Thick arrow heads point at 5-HT1B positive vessels. b nNOS and 5-HT1D double staining revealed neurons that expressed either nNOS (thin arrows) or 5-HT1D (asterisk), but also both (thick arrows). The same pattern was found for the fibers; nNOS positive (thin arrow head), 5-HT1D (two asterisks) or double stained (thick arrow head). c nNOS and 5-HT1F double staining revealed neurons that expressed either nNOS (thin arrows) or 5-HT1F (asterisk), but also both (thick arrows). d Double staining with nNOS and SNAP25 revealed that all nNOS immunoreactive neurons were also positive for SNAP25 (thin arrows). In addition, nNOS positive fibers were SNAP25 positive (arrow heads). SNAP25 immunoreactivity was found in most cells (thick arrows). e and f SV2-A (arrow heads) did not co-localize with either nNOS (thin arrows) or VIP (thin arrows). All images are shown with the same magnification