Fig. 2
Rat SPG immunohistochemistry. a PACAP-38 immunohistochemistry. PACAP-38 was found in neurons (thin arrows), fibers (arrow heads), and very intense in the SGCs (thick arrows). b nNOS was expressed in the cytoplasm of many of the neurons (thin arrows), negative neurons were also found (asterisk). In addition, immunoreactive pearl-like fibers were observed (arrow heads). c VIP immunoreactivity was disclosed in a granular manner close to the neuronal nuclei, resembling endoplasmatic reticulum staining (arrows). d Serotonin receptor 5-HT1B expression was found most neurons (thin arrows) and fibers (arrow heads), in addition to the vessel walls (thick arrows). e 5-HT1D immunoreactivity was seen in many neurons (arrows) and nerve fibers (arrow heads). f 5-HT1F immunoreactivity was seen in many neurons (arrows) and nerve fibers (arrow heads). g SNAP25 immunoreactivity was found in neurons (thin arrows) in the same granular pattern as described for VIP above. In addition, nerve fibers were immunoreactive (arrow heads). h SV2-A immunoreactivity was exclusively found in the SGCs (arrows)