Table 2 Details of primary and secondary antibodies used for IHC and WB
Name | Product code | Host | Dilution | Company | |
|---|---|---|---|---|---|
IHC | |||||
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) | 4376 | Rabbit | 1:50 | Cell Signaling Technology, Danvers, MA, USA | |
Anti IL-1 beta antibody | ab 9787 | Rabbit | 1:100 | Abcam; Cambridge, UK | |
WB | |||||
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) | 4376 | Rabbit | 1:1000 | Cell Signaling Technology, Danvers, MA, USA | |
Anti IL-1 beta antibody | ab 9787 | Rabbit | 1:500 | Abcam; Cambridge, UK |