Fig. 1

Pretreatment with methylprednisolone (MPD) suppressed IL-1β-stimulated CGRP release in trigeminal ganglia cell culture. Kruskal-Wallis test followed by Mann–Whitney U test with p-values adjusted for multiple comparisons using the Bonferroni-Holm method was used to determine significant differences. IL-1β (10 ng/ml) but not vehicle stimulation (PBS) resulted in significantly enhanced CGRP levels in the supernatant of cultured trigeminal ganglia cells after 24 h (*p = 0.031 vs. vehicle). Exposure to MPD 10 μM or 100 μM 45 min prior to stimulation with IL-1β blocked CGRP release significantly compared to pre-treatment with PBS (# p = 0.022 (10 μM) and p = 0.012 (100 μM). CGRP levels are shown in mean pg/ml ± SEM, n = 9