Table 2 Secondary antibodies used for immunohistochemistry (IH) and Western blotting (WB)
From: Kynurenic acid modulates experimentally induced inflammation in the trigeminal ganglion
Conjugate and host | Against | Dilution | Source |
|---|---|---|---|
IH | |||
FITC (goat) | Anti-rabbit | 1:100 | Cayman Chemical, Ann Arbor, MI, USA |
Texas-Red (donkey) | Anti-rabbit | 1:200 | Jackson Immunoresearch, West Grove, PA, USA |
DyLight 549 (donkey) | Anti-mouse | 1:200 | Jackson Immunoresearch, West Grove, PA, USA |
DyLight 488 (donkey) | Anti-sheep | 1:200 | Jackson Immunoresearch, West Grove, PA, USA |
Alexa 488 (donkey) | Anti-goat | 1:400 | Invitrogen, La Jolla, CA, USA |
WB | |||
HRP-conjugated | Anti-rabbit | 1:2000 | Cell Signaling Technology, Danvers, MA, USA |
HRP-conjugated | Anti-mouse | 1:2000 | Cell Signaling Technology, Danvers, MA, USA |