Table 1 Details of primary antibodies used for immunohistochemistry (IH) and Western blotting (WB)
From: Kynurenic acid modulates experimentally induced inflammation in the trigeminal ganglion
Name | Product code | Host | Dilution | Source | |
|---|---|---|---|---|---|
IH | WB | ||||
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) | 4376 | Rabbit | 1:50 | 1:1000 | Cell Signaling Technology, Danvers, MA, USA |
Phospho-p38 MAPK (Thr180/Tyr182) | 9216 | Mouse | 1:400 | 1:2000 | Cell Signaling Technology, Danvers, MA, USA |
Phospho-SAPK/JNK (Thr183/Tyr185) | 9255 | Mouse | 1:400 | 1:2000 | Cell Signaling Technology, Danvers, MA, USA |
Anti-NFκB p65 (phosphor S529) | ab97726 | Rabbit | 1:100 | 1:1000 | Abcam; Cambridge, UK |
CaMKII | ab52476 | Rabbit | 1:100 | 1:20000 | Abcam; Cambridge, UK |
DREAM (FL-214) | sc-9142 | Rabbit | 1:250 | 1:500 | Santa Cruz Biotech, Santa Cruz, CA, USA |
Anti-Kir4.1 | APC-035 | Rabbit | 1:1000 | -- | Alomone Labs Ltd., Jerusalem, Israel |
Anti-β-actin | Sc-47778 | Mouse | -- | 1:5000 | Santa Cruz Biotech, Santa Cruz, CA, USA |
CGRP | ab81887 | Mouse | 1:100 | -- | Abcam; Cambridge, UK |
CLR | 132 | Sheep | 1:100 | -- | Merck & Co., Inc |
RAMP1 | 844 | Goat | 1:100 | -- | Merck & Co., Inc |