Table 2 Details of secondary antibodies used for IHC and WB
Conjugate and host | Against | Dilution | Company | |
|---|---|---|---|---|
IHC | ||||
FITC (goat) | Anti-rabbit | 1:100 | Cayman Chemical, Ann Arbor, MI, USA | |
Alexa 594 (goat) | Anti-mouse | 1:100 | Invitrogen, CA, USA | |
WB | ||||
HRP-conjugated | Anti-rabbit | 1:2000 | Cell Signaling Technology, Danvers, MA, USA | |
HRP-conjugated | Anti-mouse | 1:2000 | Cell Signaling Technology, Danvers, MA, USA |