Table 1 Details of primary antibodies used for IHC and WB
Name | Product code | Host | Dilution | Company | |
|---|---|---|---|---|---|
IHC | |||||
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) | 4376 | Rabbit | 1:50 | Cell Signaling Technology, Danvers, MA, USA | |
Anti IL-1 beta antibody | ab 9787 | Rabbit | 1:100 | Abcam; Cambridge, UK | |
Calcitonin-gene related peptide, polyclonal | B-47-1 | Rabbit | 1:800 | Europroxima, Arnhem, Netherlands | |
Phospho-p38 MAPK (Thr180/Tyr182) | 9216 | Mouse | 1:200 | Cell Signaling Technology, Danvers, MA, USA | |
Phospho-SAPK/JNK (Thr183/Tyr185) | 9252 | Mouse | 1:200 | Cell Signaling Technology, Danvers, MA, USA | |
Anti TNF-alpha antibody | ab66579 | Rabbit | 1:200 | Abcam; Cambridge, UK | |
Anti IL-6 antibody | ab6672 | Rabbit | 1:100 | Abcam; Cambridge, UK | |
Anti-Glutamine Synthetase Antibody, Clone GS-6 | MAB302 | Mouse | 1:100 | Merck Milipore, Darmstadt, Germany | |
WB | |||||
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) | 4376 | Rabbit | 1:1000 | Cell Signaling Technology, Danvers, MA, USA | |
Anti IL-1 beta antibody | ab 9787 | Rabbit | 1:500 | Abcam; Cambridge, UK | |
Anti-Calcitonin Gene Related Peptide | C8198 | Rabbit | 1:250 | Sigma-Aldrich, St. Louis, MO, USA | |
Anti β-actin | Sc-477778 | Mouse | 1:5000 | Santa Cruz Biotech, Santa Cruz, CA, USA | |
GAPDH (D16H11) XP mAb | 5174 | Rabbit | 1:1000 | Cell Signaling Technology, Danvers, MA, USA |