Fig. 4
IL-1β immunohistochemistry on the TG a. Negative control showing no immunoreactivity. b. IL-1β staining in fresh animals. Immunoreactive neuronal nuclei could be detected (thick arrow). In addition, an intracytoplasmatic granular staining was seen (thin arrow). c. 24 h vehicle group showed a similar pattern as for fresh animals. Inserts immunoreactive nerve fibers. d-i. Immunoreactivity was detected in the neuronal nuclei, but no nucleolei in the CFA and IS treated groups (thick arrows, d-i). In the cytoplasm immunoreactivity was detected in a granular manner (thin arrows, d-i). In the 7 days CFA and IS groups, increased immunoreactivity was seen as a condensed, homogeneous material close to the cell membrane (i, arrow heads)