Fig. 4

IL1β immunohistochemistry. a Almost all neurons in the fresh specimens displayed IL1β immunoreactivity, which appeared as granules in the cytoplasm. This pattern was also found in the saline incubated groups. The groups incubated with U0126 or BoNT-A showed none or little IL1β immunoreactivity. b Fluorescence intensity measurements confirmed these findings. Bars indicate SD