Fig. 2

CGRP immunohistochemistry. a Fresh TG contained both CGRP positive and negative neurons. The immunoreactivity was confined to the cytoplasm in a granular pattern, resembling staining of the endoplasmatic reticulum, and in some of the fibers. In TG incubated in medium containing saline, the number of neurons immunoreactive to CGRP seemed to be increased. The immunoreactivity was often spread in a granular matter in the entire cytoplasm as observed in the fresh TG. In the specimens incubated with U0126 or BoNT-A, the number of positive neurons increased and the immunoreactivity was found in the entire cytoplasm in almost all neurons. b To illustrate the microscopical findings, fluorescence intensity measurements were performed. The results confirm these findings; a tendency of intensity increase after 24 hrs of incubation with NaCl, U0126 or BoNT-A. Negative column is the same as in SNAP-25 diagram since the same secondary antibody is used in both experiments. Bars indicate SD