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EHMTI-0092. Activation of the 5-HT2B receptor induces dural plasma protein extravasation in a mouse migraine model
The Journal of Headache and Pain volume 15, Article number: F11 (2014)
Migraine attacks originate in the meninges which are densely innervated by trigeminal nerve fibers. Upon stimulation, endothelial cells of dural blood vessels secrete nitric oxide and as a consequence, neuropeptides are released from trigeminal nerve fibers. This, in turn, leads to meningeal plasmaproteinextravasation (PPE) and vasodilation. Inflammatory components such as PPE and vasodilation serve as indicators for migraine attacks in animal models. A guinea pig migraine model was described in which the partial 5-HT2B/2C receptor agonist meta-Chlorphenylpiperazine (mCPP) is applied to induce PPE in the dura mater. We have transferred this model to mice.
Characterize the role of the 5-HT2B in a mouse migraine model.
Tissue accumulation of the tracer Evans Blue is measured to quantify the extent of the PPE.
Mice are only responsive to mCPP after several weeks of hypoxia. The induction of PPE in hypoxic mice is blocked by the specific 5-HT2B receptor antagonist BF-1.
The 5-HT2B receptors play a crucial role in the induction of migraine symptoms in hypoxic mice.
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Hunfeld, A., Kremser, M., Segelcke, D. et al. EHMTI-0092. Activation of the 5-HT2B receptor induces dural plasma protein extravasation in a mouse migraine model. J Headache Pain 15, F11 (2014). https://doi.org/10.1186/1129-2377-15-S1-F11
- Nitric Oxide
- Migraine Attack
- Dura Mater
- Evans Blue