Fig. 2

Volcano plots showing up/down regulation of cytokine genes as response to different treatments of the trigeminal ganglia body tissue. a Fresh versus organ culture, huge significant upregulation of the pro-inflammatory cytokines IL-6 and lif can be seen; furthermore, a number of cytokines are down regulated as response to organ culture. b Organ culture versus CGRP treatment, additional significant upregulation of a number of cytokines. c Organ culture versus CGRP_8–37 treatment: significant down regulation of the pro-inflammatory cytokine IL-18; otherwise no effect of this treatment. d Organ culture versus U0126 treatment: significant upregulation of the anti-inflammatory cytokine IL-10; otherwise no effect of this treatment. e organ culture versus SB202190 treatment: little effect of this treatment. f Organ culture versus SP600125 treatment: significant down regulation of a number of cytokines indicating an anti-inflammatory effect of the JNK inhibitor. Red circles indicate upregulation and green circles indicate down regulation. Values are considered statistically significant when fold change is larger than 3 and P < 0.05 (above line) (colour figure online)